Foreword

Introduction

1Scope

2Normative references

3Terms and definitions

4Principle

5Reagents

5.1Extraction buffer

5.2Sample buffer

6Apparatus

7Sampling

8Preparation

8.1Protein content of test sample

8.2Preparation of test portion

9Procedure

9.1Operating conditions

9.1.1Running conditions for capillary electrophoresis (CE)

9.1.2Injection

9.2Testing instrument suitability

9.3Qualitative analysis

9.3.1Analysis

9.3.2Peak identification

9.4Quantitative analysis

9.4.1Analysis

9.4.2Blank sample

9.4.3Measurements

9.4.4Peak identification

9.4.5Integration of peak areas

9.4.6Calibration

9.4.6.1Reference sample

9.4.6.2Calculation of the response factors

10Calculation and expression of results

10.1Qualitative analysis

10.2Quantitative analysis

10.2.1Calculation

10.2.2Expression of test results

11Precision

11.1Interlaboratory test

11.2Repeatability

11.3Reproducibility

12Test report

Annex AExamples of electropherograms (informative)

Annex BInterlaboratory trials (informative)

Bibliography