Foreword

9Procedure

9.1Preparation of test sample

9.2Liquid-liquid extraction

9.2.1Separation funnel

9.2.2Ludwig extraction

9.3Clean-up

9.3.1General

9.3.2Diatomaceous earth-sulfuric acid column (optional)

9.3.2.1Preparation of the column

9.3.2.2Running the column

9.3.3Gel permeation (size exclusion) chromatography/porous styrene divinylbenzene beads-column (optional)

9.3.3.1Preparation of the column

9.3.3.2Running the column

9.3.4Multilayer columns (obligatory)

9.3.4.1Preparation of a large-volume multilayer column

9.3.4.2Running the large-volume multilayer column

9.3.4.3Two-step wet column

9.3.4.3.1Preparation of the column

9.3.4.3.2Running the column

9.3.5Alumina column (optional)

9.3.5.1Preparation of the column

9.3.5.2Running the column

9.4Preparation of GC solution and GC procedure

9.5Calculation and quantitation procedure