DIN EN 16618:2015-06

This European Standard specifies a method for the determination of acrylamide in bakery ware such as bread, toasted bread, crisp bread, butter cookies, and biscuits, as well as potato products such as potato chips, potato crisps, and potato pan cake and roasted coffee, by liquid chromatography in combination with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples, ranging from 14,3 μg/kg to 9 083 μg/kg. It was developed at the Swedish National Food Administration and validated in a study organized by the Directorate General Joint Research Centre (DG JRC), Swedish National Food Administration and the Nordic Committee on Food Analysis (NMKL). The limit of quantification (LOQ) depends on the type of instrument used and on the actual performance of the instrument. The majority of the laboratories participating in the validation study were able to determine acrylamide in a butter cookie sample at a level of 14,3 μg/kg. Thus, the validation by interlaboratory study showed that LOQ can be expected to be in the range between below 15 μg/kg and 30 μg/kg. This document (EN 16618:2015) has been prepared by Technical Committee CEN/TC 275 "Food analysis - Horizontal methods" the secretariat of which is held by DIN (Germany). The responsible national standardization committee is Working Committee NA 057-01-14 AA "Prozesskontaminanten" ("Process contaminants") at DIN Standards Committee Food and Agricultural Products (NAL).