DIN EN ISO 11816-2:2025-08

This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in cheese. This method is applicable to soft cheeses, semi-hard and hard cheeses provided that the mould is only on the surface of the cheese and not also in the inner part (for example, blue veined cheeses). For large hard cheeses, specific conditions of sampling apply (see Clause 7). The instrument used for the determination of ALP can read activities in the supernatant up to 7 000 milliunits per litre (mU/kg). This document (EN ISO 11816-2:2024) has been prepared by Technical Committee ISO/TC 34 "Food products" in collaboration with CEN/TC 302 "Milk and milk products - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). The responsible national standardization committee is Working Committee NA 057-05-13 AA "Milch und Milchprodukte - Probenahme- und Untersuchungsverfahren" ("Milk and milk products - Methods of sampling and analysis") at DIN Standards Committee Food and Agricultural Products (NAL).