DIN 58988:2024-04

DIN 58988 describes a reference method for determining the number and size distribution and for assessing the substructures of human Von Willebrand factor multimers. Von Willebrand disease is caused by congenital or acquired quantitative and/or functional disorders of the von Willebrand factor. It is one of the most common congenital or acquired coagulation disorders with an increased tendency to bleed. The analysis of the Von Willebrand factor multimers for the precise determination of the type or subtype of Von Willebrand disease consists of the electrophoresis of the sample in an agarose gel, either the gel fixation or the transfer of the proteins onto a membrane, and the immunodetection of the protein. This document has been prepared by Working Committee NA176-08-04 AA "Hämostaseologie" ("Haemostaseology") at DIN Standards Committee Health Technologies (NAGesuTech).