DIN EN 16160:2012-05

The standard DIN EN 16160 is applicable to the quantitative analysis of (bound and free) hydrocyanic acid (HCN) in feed materials of plant origin and compound feed by High Performance Liquid Chromatography (HPLC). The method is validated from 10 mg HCN/kg to 350 mg HCN/kg. When the method is used outside this range it should be validated at least within the laboratory. A limit of quantification of 2 mg HCN/kg should normally be obtained. Hydrocyanic acid occurs in feed as cyanoglycosides. Cyanoglycosides are degraded by enzymes generating cyanide. The European Standard has been prepared by CEN/TC 327 "Animal feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). The collaboration of DIN for the field of responsibility of CEN/TC 327 is performed through national mirror committee, Working Committee NA 057-03-03 AA "Futtermittel" ("Animal feeding stuffs") at NAL.