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This European Standard specifies a method to assess the ability of biocide-containing coatings to prevent the germination of conidiospores of certain fungi known to be capable of proliferating in fuel systems for aerospace applications. This standard EN 4159 has been prepared by ASD-STAN, Technical Section "Non-metallic materials", with the participation of German experts of the Coatings and Coating Materials Standards Committee (NAB) and the Aerospace Standards Committee (NL).