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This document specifies a fluorimetric method for the determination of alkaline phosphatase (ALP) (EC 3.1.3.1) activity in raw and heat-treated whole milk, semi-skimmed milk, skimmed milk and flavoured milks. This method is applicable to milk and milk-based drinks from cows, sheep and goats. It is also applicable to milk powder after reconstitution. The instrument used for the determination of ALP can read activities up to 7 000 milliunits per litre (mU/l). If the activity is higher than 7 000 mU/l, it is diluted with ALP-free milk so as to obtain a measurement not higher than 7 000 mU/l. This document (EN ISO 11816-1:2024) has been prepared by Technical Committee CEN/TC 302 "Milk and milk products - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). The responsible national standardization committee is Working Committee NA 057-05-13 AA "Milch und Milchprodukte - Probenahme- und Untersuchungsverfahren" ("Milk and milk products - Methods of sampling and analysis") at DIN Standards Committee Food and Agricultural Products (NAL).
This document replaces DIN EN ISO 11816-1:2014-03 .