DIN EN ISO 6888-1:2024-03

This document, ISO 6888-2 and ISO 6888-3 describe three horizontal methods for the detection and enumeration of coagulase-positive staphylococci among which enterotoxinogenic strains are encountered. It is mainly concerned with Staphylococcus aureus, but also with S. intermedius and certain strains of S. hyicus. For the purposes of this document, the confirmation of typical and atypical colonies is based on a positive coagulase reaction, but it is recognized that some strains of Staphylococcus aureus give weakly positive coagulase reactions. These latter strains can be confused with other bacteria but they can be distinguished by the use of additional tests not included in this document, such as tests for sensitivity to lysostaphin, and production of haemolysin, thermostable nuclease and acid from mannitol. This document is applicable to: - products intended for human consumption; - products intended for animal feeding; - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage. The responsible German standardization committee is Working Committee NA 057-01-06 AA "Mikrobiologie der Lebensmittelkette" ("Microbiology of the food chain") at DIN Standards Committee Food and Agricultural Products (NAL.